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| Call for Papers>>Final Program |
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| Abstract of admission as follows: |
| Digital In-line Holographic Microscopy and Tomography |
| H.J. Kreuzer
PhD from the University of Bonn, Germany,professor of Theoretical Physics at Dalhousie University ,External Member of the Max-Planck-Society (Germany),
fellow of the Royal Society of Canada |
| Abstract |
| Digital In-line Holographic Microscopy and Tomography 4-D imaging and tracking of micro-structures and organisms in microfluidics and biology H.J. Kreuzer, Department of Physics and Atmospheric Science, Dalhousie University, Halifax, NS B3H 3J5 Canada Digital in-line Holography with numerical reconstruction has been developed into a new microscopy, specifically for microfluidic and biological applications, that routinely achieves both lateral and depth resolution at the submicron level in 3-D imaging. This is demonstrated for diverse objects such as suspensions of microspheres and biological samples (cells, algae, protozoa, bacteria). The motion of (many) living cells in water can be tracked in 3-D at subsecond rates. Microfluidic applications include sedimentation of suspensions, fluid motion around micron-sized objects in channels, motion of spheres and formation of bubbles. I will show results with a new method called Immersion DIHM which effectively does holography in the UV. I will also introduce a submersible version of the microscope that allows the in-situ study of marine life in real time in the ocean and show images and films obtained in sea trials. Further on the instrumental side, I will present and demonstrate the capabilities of a mini-DIHM designed as an add-on to an inverted microscope such as the Zeiss Axiovert 25 and 40 and the Leica DM IRE2 and similar instruments. Lastly, I will outline a new approach called Digital In-line Holographic Tomography. |
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